Main TopicsWhat is major groove and minor groove in DNA?
Table of Contents
- What is major groove and minor groove in DNA?
- How do you determine the major and minor grooves in DNA?
- What drives proteins into the major or minor grooves of DNA?
- How do the major and minor grooves in a DNA compare to those in B-DNA?
- What are the 3 types of DNA?
- What causes the major groove in DNA?
- What statement about the major and minor grooves is true?
- What proteins are associated with DNA?
- Why are the two grooves of B DNA different widths?
- Why is B form DNA most stable?
- When do major and minor grooves occur in DNA?
- Why do proteins bind to the major grooves of DNA?
- Why are there grooves in the DNA helix?
- Why are the grooves in double stranded DNA symmetrical?
What is major groove and minor groove in DNA?
The major groove occurs where the backbones are far apart, the minor groove occurs where they are close together. The grooves twist around the molecule on opposite sides. Certain proteins bind to DNA to alter its structure or to regulate transcription (copying DNA to RNA) or replication (copying DNA to DNA).
How do you determine the major and minor grooves in DNA?
0:271:40DNA major and minor grooves - YouTubeYouTubeStart of suggested clipEnd of suggested clipYou'll always remember the correct orientation for the left side of a major groove. Looking again atMoreYou'll always remember the correct orientation for the left side of a major groove. Looking again at the left backbone. We can see that in the minor groove the deoxyribose.
What drives proteins into the major or minor grooves of DNA?
entropic forces Since the entropic forces driving protein binding into the minor groove are a consequence of displacing water ordered by the regular arrangement of polar contacts, they cannot be regarded as hydrophobic.
How do the major and minor grooves in a DNA compare to those in B-DNA?
How do the major and minor grooves in A-DNA compare to those in B-DNA? The major groove in B-DNA is much larger than the minor groove. In A-DNA, both grooves are about the same size.
What are the 3 types of DNA?
Three major forms of DNA are double stranded and connected by interactions between complementary base pairs. These are terms A-form, B-form,and Z-form DNA.
What causes the major groove in DNA?
Double-helical nucleic acid molecules contain two grooves, called the major groove and the minor groove. These grooves arise because the glycosidic bonds of a base pair are not diametrically opposite each other (Figure 27.7).
What statement about the major and minor grooves is true?
The major and minor grooves are important for DNA binding proteins to attach to the DNA. The major groove is formed from pyrimidines and the minor groove is formed from purines. The major groove runs in the 5' to 3' direction and the minor groove runs in the 3' to 5' direction. None of these statements are TRUE.
What proteins are associated with DNA?
Histones are a family of basic proteins that associate with DNA in the nucleus and help condense it into chromatin. Nuclear DNA does not appear in free linear strands; it is highly condensed and wrapped around histones in order to fit inside of the nucleus and take part in the formation of chromosomes.
Why are the two grooves of B DNA different widths?
The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.
Why is B form DNA most stable?
It is known that the stability of the double helical structure of B-DNA is supplied by the hydrogen bonds as proposed by Watson and Crick3 and by the stacking interactions. However, the relative importance of both stabilizing interactions as well as how they interfere with each other is largely unknown.
When do major and minor grooves occur in DNA?
The major groove occurs where the backbones are far apart, the minor groove occurs where they are close together. The grooves twist around the molecule on opposite sides. Certain proteins bind to DNA to alter its structure or to regulate transcription (copying DNA to RNA) or replication (copying DNA to DNA). It is easier for these
Why do proteins bind to the major grooves of DNA?
Certain proteins bind to DNA to alter its structure or to regulate transcription (copying DNA to RNA) or replication (copying DNA to DNA). It is easier for these DNA binding proteins to interact with the bases (the internal parts of the DNA molecule) on the major groove side because the backbones are not in the way.
Why are there grooves in the DNA helix?
The grooves result from geometry of the Watson-Crick base-pair namely the Purines (Adenine and Guanine) and the Pyrimidines (Thymine and Cytosine); which then results in a Major and Minor groove. They arise due to the orientation of the base-pairs across the helix. The base-pairs we observe in the DNA are A:T, T:A, G:C, or C:G.
Why are the grooves in double stranded DNA symmetrical?
The relevant property is what they call "opening". From the biochemistry textbook by Berg: To explain in words, if the glycosidic bonds (which attach the nucleic base to the sugar in the backbone) stuck straight out at 90 angles on both sides, then the grooves in double-stranded DNA would be symmetrical.
