How does SFC chromatography work?

How does SFC chromatography work?

How does SFC chromatography work?

Supercritical fluid chromatography (SFC) uses highly compressed gas above its critical temperature and pressure instead of an organic solvent as the solvent phase. The SFC detecting systems are those commonly used in GC. ... Gases such as carbon dioxide, nitrous oxide, and ammonia are commonly used.

Which compound can be detected by SFC?

Carbon dioxide is one chemical that can be easily converted into a supercritical fluid for use in SFC. The formation of supercritical fluid carbon dioxide occurs at or above a temperature of 31.1° C and at or above a pressure of 73.9 bar (72.9 atm).

Why is co2 used in SFC?

Mobile Phases The most common supercritical fluid which is used in SFC is carbon dioxide because its critical temperature and pressure are easy to reach. Additionally, carbon dioxide is low-cost, easy to obtain, inert towards UV, non-poisonous and a good solvent for non-polar molecules.

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